SNU-449（人肝癌細(xì)胞）
CBP60217
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| I. General information | |
| Synonyms: | SNU-449 |
| Background: | SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Species: | Homo sapiens, human |
| Tissue: | liver， 52 years，Asian |
| Disease: | grade II-III/IV, hepatocellular carcinoma |
| Gender: | male， |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 36 hrs |
| Culture Medium: |
RPMI-1640+10%FBS SNU-449完全培養(yǎng)基，# CBP60217M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type. The cultured cells contain a single or double nucleus. ATCC confirmed this cell line is positive for the presence of Hepatitis B viral DNA sequences. For more information, please contact Cobioer (4008-750-250). |
