SNU-398（人肝癌細(xì)胞）
CBP60215
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| I. General information | |
| Synonyms: | SNU-398 |
| Background: | SNU-398 was derived in 1990 by J.-G. Park and associates from an anaplastic hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum. |
| Species: | Homo sapiens, human |
| Tissue: | liver |
| Disease: | hepatocellular carcinoma |
| Gender: | male, 42 years, Asian |
| Morphology: | epithelial |
| Growth Mode: | mixed adherent and floating |
| Doubling Time: | 39 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 13 D13S317: 11 D16S539: 10,14 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 11 vWA: 17,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS SNU-398完全培養(yǎng)基，# CBP60215M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Blood Type O; Rh + |
| Comments: | Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was trabecular type. The cultured cells are multinucleated, and maintain the production of intracytoplasmic hyaline globules as seen in the original tumor. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. For more information, please contact Cobioer (4008-750-250). |
