NCI-H1623（人非小細胞肺癌細胞）
CBP60059
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產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. General information | |
| Synonyms: | NCI-H1623 |
| Background: | The line was established in April 1987. |
| Species: | Homo sapiens, human |
| Tissue: | lung; derived from metastatic site: lymph node |
| Disease: | stage 3B, adenocarcinoma; non-small cell lung cancer |
| Gender: | male, 58 years, Caucasian,The patient was a smoker. |
| Growth Mode: | adherent, single cells and loosely attached clusters |
| Culture Medium: |
DMEM: F12(1:1)+5%FBS:
• 0.02 mg/ml insulin
• 0.01 mg/ml transferrin
• 25 nM sodium selenite (final conc.)
• 50 nM Hydrocortisone (final conc.)
• 1 ng/ml Epidermal Growth Factor (do not filter)
• 0.01 mM ethanolamine (final conc.)
• 0.01 mM phosphorylethanolamine (final conc.)
• 100 pM triiodothyronine (final conc.)
• 0.5% (w/v) bovine serum albumin (final conc.)
• 10 mM HEPES
• 0.5 mM sodium pyruvate (final conc.)
• extra 2mM L-glutamine (for final conc. of 4.5mM)
We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
