BV-2（C57BL/6小鼠腦小膠質(zhì)細(xì)胞(永生化)）
CBP60922
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| I. General information | |
| Synonyms: | BV-2 |
| Background: | This cell line was transformed by retroviruses |
| Species: | Mus muscullus |
| Tissue： | Brain, microglial cells |
| Morphology: | morphology microglial |
| Subcultivation ratio: : | 1:5 to 1:10 |
| Culture Medium: |
RPMI-1640+10%FBS BV-2完全培養(yǎng)基，# CBP60922M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comment | The BV-2 cells express the nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level; the BV-2 cells have morphological, phenotypical and functional markers of macrophages To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. |
