hFOB 1.19（人SV40轉(zhuǎn)染成骨細(xì)胞）
CBP60724
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| I. General information | |
| Synonyms: | hFOB 1.19 |
| Background: | This line was established by transfection of limb tissue obtained from a spontaneous miscarriage with the temperature sensitive expression vector pUCSVtsA58 and the neomycin resistance expression vector pSV2-neo. Clones were selected in the presence of 0.6 mg/mL G418. |
| Species: | Homo sapiens, human |
| Tissue: | bone |
| Gender: | fetus |
| Morphology: | osteoblast; SV40 large T antigen transfected |
| Growth Mode: | adherent |
| Doubling Time: | 36 hours |
| DNA Profile: | Amelogenin: X CSF1PO: 10,13 D13S317: 11,12 D16S539: 9,13 D5S818: 11,12 D7S820: 8,10 THO1: 7,9.3 TPOX: 11 vWA: 16,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM:F12(1:1)+0.3 mg/ml G418+10%FBS hFOB 1.19完全培養(yǎng)基，# CBP60724M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | SV40 T antigen |
| Genes Expressed: | alkaline phosphatase |
| Cellular Products: | alkaline phosphatase |
| Comments: | Cells grown at a permissive temperature of 33.5°C exhibit rapid cell division (Doubling time of 36 hrs), whereas little cell division occurs at a restrictive temperature of 39.5°C (Doubling time of 96 hrs). The cells have the ability to differentiate into mature osteoblasts expressing the normal osteoblast phenotype. At the restrictive temperatures, cell division is slowed, differentiation increases, and a more mature osteoblast phenotype is produced. For more information, please contact Cobioer (4008-750-250). |
