OCI-LY19（人B細(xì)胞淋巴瘤）
CBP60621
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| I. General information | |
| Synonyms: | OCI-LY19 |
| Background: | established from the bone marrow of a 27-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL) (diffuse large cell lymphoma, DLCL, stage 4B, at relapse) in 1987 |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | B cell lymphoma |
| Gender: | N/A |
| Morphology: | single round cells growing in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
80% MEM α (GIBCO 12561-056 ) + 20% FBS OCI-LY19完全培養(yǎng)基，# CBP60621M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD37 -, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 48(46-52)<2n>X, -X, +6, +6, +8, t(4;8)(q3?2;q?24), del(6)(q15)x2, r(8)(??), t(14;18)(q32;q21), add(18)(q23) - carries t(14;18) effecting IGH-BCL2 juxtaposition |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
