JVM-3（人慢性髓細(xì)胞樣白血病細(xì)胞）
CBP60618
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| I. General information | |
| Synonyms: | JVM-3 |
| Background: | established from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells express mRNA of the proto-oncogenes BCL2 and BCL3 |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | chronic B cell leukemia |
| Gender: | N/A |
| Morphology: | lymphoblastoid cells growing singly or in clumps in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS JVM-3完全培養(yǎng)基，# CBP60618M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | originally confirmed as human with IEF of AST, MDH, NP, later by DNA-fingerprinting |
| Cytogenetics: | human near tetraploid karyotype without clear mode - 87-93<4n>XXYY, +3, -7, -8, -10, +12, +12, -14, -19, -19, -20, -20 - appears to be derivate of original hyperdiploid line which exhibited trisomy 12 |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
