CX3CR1/β-Arrestin/CHO
CBP71409
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| I. Background | |
| CX3C 基序趨化因子受體 1 (CX3CR1)，也稱(chēng)為分形因子受體或 G 蛋白偶聯(lián)受體 13 (GPR13)，是 G 蛋白偶聯(lián)受體 1 (GPCR1) 家族的跨膜蛋白，也是 CX3C 趨化因子受體亞 家族中唯一已知的成員。它是一種高度選擇性的表面受體，通常在單核細(xì)胞、CD4 T 細(xì)胞、 CD8 T 細(xì)胞、NK 細(xì)胞和 γδ 細(xì)胞上表達(dá)。CX3CL1 是一種獨(dú)特的趨化因子，可與其唯一受 體 CX3CR1 結(jié)合。 CX3CR1-CX3CL1 細(xì)胞內(nèi)信號(hào)級(jí)聯(lián)負(fù)責(zé)調(diào)節(jié)細(xì)胞活性，使其達(dá)到更高的活性狀態(tài)，如 存活、遷移和增殖等。 |
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| II. Description | |
| CX3CR1 β-Arrestin CHO 報(bào)告基因藥靶模型很好的模擬了體內(nèi) CX3CR1/β-Arrestin 的信 號(hào)轉(zhuǎn)導(dǎo)過(guò)程，原理見(jiàn)下圖所示。 | |
 Figure 1. CX3CR1 β-Arrestin CHO 細(xì)胞模型原理圖
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| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5ug/ml puromycin+5ug/ml blastcidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CX3CR1 receptor |
| Assay Format: | β-arrestin recruitment |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 2. Recombinant CX3CR1/β-Arrestin/CHO constitutively expressing CX3CR1. |
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Figure 3. Dose response of Human CX3CL1 in CX3CR1 β-Arrestin CHO-K1 Cell Line (C13). |
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